Ethyl 2-[(2-aminoacetyl)amino]acetate hydrochloride is primarily used in organic synthesis and pharmaceutical research. It serves as a key intermediate in the preparation of peptides and other biologically active compounds. The compound is particularly valuable in the development of drug candidates, where its structure allows for the introduction of specific functional groups that enhance biological activity. Additionally, it is utilized in the study of enzyme inhibitors and receptor ligands, aiding in the understanding of biochemical pathways and the design of therapeutic agents. Its stability and reactivity make it a versatile reagent in medicinal chemistry and drug discovery processes.