N-Fmoc-glycylglycine is widely used in peptide synthesis as a building block. It serves as a protected dipeptide, where the Fmoc group acts as a temporary protecting group for the amino function during solid-phase peptide synthesis (SPPS). This compound is particularly valuable in the stepwise assembly of peptides, allowing for the precise control of sequence and structure. It is commonly employed in the production of biologically active peptides, therapeutic agents, and research peptides. Additionally, it finds application in the development of peptide-based drugs, vaccines, and diagnostic tools due to its compatibility with automated synthesizers and efficient deprotection process. Its use ensures high purity and yield in peptide manufacturing.