L-Pyroglutamyl-L-alanine is primarily used in the field of biochemistry and peptide research. It serves as a dipeptide model in studies to understand peptide structure, stability, and interactions. This compound is often utilized in enzymatic studies to investigate the activity and specificity of peptidases and proteases. Additionally, it finds application in the development of peptide-based drugs and therapeutic agents, where its properties help in understanding the pharmacokinetics and pharmacodynamics of peptide molecules. In cosmetic formulations, it is sometimes incorporated for its potential skin-conditioning properties, contributing to the improvement of skin texture and hydration. Furthermore, it is used in nutritional science as a component in specialized diets and supplements aimed at promoting muscle recovery and overall health.