Cyclo(-Ala-Ala) finds its primary application in the field of peptide research and drug development. It is often utilized as a model compound to study the conformational properties of cyclic dipeptides, which are essential for understanding peptide folding and stability. This compound is also employed in the synthesis of more complex peptide structures, serving as a building block in the creation of biologically active molecules. Additionally, Cyclo(-Ala-Ala) is used in the development of peptidomimetics, which are designed to mimic the structure and function of natural peptides for therapeutic purposes. Its role in exploring enzyme inhibition and receptor binding further highlights its significance in medicinal chemistry.