Glycyl-L-leucine is widely used in biochemical research as a dipeptide model to study peptide bond formation, hydrolysis, and enzyme specificity. It serves as a substrate for proteolytic enzymes, aiding in the investigation of enzyme kinetics and mechanisms. In the field of nutrition, it is utilized to explore peptide absorption and metabolism in the human body, providing insights into amino acid delivery systems. Additionally, it is employed in the development of synthetic peptides and peptidomimetics for pharmaceutical applications, particularly in drug design targeting specific enzymes or receptors. Its role in cell culture media supports the growth and study of microorganisms, contributing to advancements in microbiology and biotechnology.