Z-Pro-Pro-OH is primarily used in peptide synthesis and biochemical research. It serves as a building block for constructing more complex peptide structures, particularly in studies involving proline-rich sequences. Its application extends to investigating enzyme specificity, protein folding, and peptide interactions, as proline residues play a critical role in secondary structure formation. Additionally, it is utilized in the development of peptide-based drugs and inhibitors, especially those targeting proline-specific enzymes like prolyl oligopeptidase. Its stability and compatibility with solid-phase peptide synthesis make it a valuable tool in organic and medicinal chemistry.