L-Threonyl-L-leucine is primarily used in the field of biochemistry and pharmaceutical research. It serves as a dipeptide that can be utilized in the study of peptide synthesis and protein engineering. Researchers often employ it to investigate enzymatic reactions and peptide bond formation, which are crucial for understanding metabolic pathways and developing therapeutic agents. Additionally, it may be used in the development of nutritional supplements or bioactive peptides aimed at enhancing muscle recovery and overall health. Its role in mimicking natural peptides makes it valuable for studying biological processes and designing novel drugs.