Used as a crosslinking agent in biochemical and bioconjugation applications. It is particularly effective in linking proteins, peptides, or other biomolecules through primary amino groups via its sulfo-NHS ester functionalities, introducing a central disulfide bridge that enables the study of molecular interactions or the creation of stable, reducible complexes. Commonly employed in antibody labeling, protein-protein crosslinking, and immobilization of biomolecules on solid supports. Its water-soluble nature, conferred by sulfonate groups, makes it suitable for use in aqueous environments, ensuring compatibility with biological systems. Additionally, it is utilized in the development of diagnostic assays and therapeutic conjugates due to its ability to form cleavable disulfide bonds, and it can also participate in thiol-disulfide exchange reactions.