Diprotin A TFA is primarily used in biochemical research as a selective inhibitor of dipeptidyl peptidase IV (DPP-IV). This enzyme plays a significant role in glucose metabolism, making the compound valuable in studies related to diabetes and metabolic disorders. Researchers utilize it to investigate the mechanisms of DPP-IV and its impact on peptide hormones like GLP-1, which regulates insulin secretion. Additionally, it is employed in cell-based assays to study immune regulation, as DPP-IV is involved in T-cell activation and immune responses. Its application extends to drug discovery, where it serves as a tool to screen and develop potential therapeutic agents targeting DPP-IV-related pathways.