Monodispersed magnetite microspheres are widely used in biomedical applications due to their magnetic properties and uniform size. They are employed in magnetic resonance imaging (MRI) as contrast agents to enhance image clarity for diagnostic purposes. In drug delivery systems, these microspheres enable targeted therapy by guiding drugs to specific sites in the body using an external magnetic field. They are also utilized in cell separation and sorting processes, where their magnetic nature allows for efficient isolation of specific cell types from complex mixtures. Additionally, they play a role in hyperthermia treatment for cancer, where they generate heat under an alternating magnetic field to destroy tumor cells. In environmental applications, they are used for wastewater treatment to remove heavy metals and other contaminants through magnetic separation. Their uniformity and magnetic responsiveness make them valuable in various scientific and industrial fields.