Monodispersed magnetite microspheres are widely used in biomedical applications due to their magnetic properties and uniform size. They are employed in magnetic resonance imaging (MRI) as contrast agents to enhance the visibility of internal body structures. In targeted drug delivery systems, these microspheres are utilized to transport therapeutic agents directly to specific sites, such as tumors, by guiding them with an external magnetic field. Additionally, they play a crucial role in cell separation and sorting processes, where they are coated with specific antibodies to bind and isolate target cells from complex mixtures. In environmental applications, these microspheres are used for wastewater treatment to remove heavy metals and other contaminants through magnetic separation. Their uniformity and magnetic responsiveness make them valuable in various scientific and industrial fields.