Monodispersed magnetite microspheres are widely used in biomedical applications due to their magnetic properties and uniform size. They are employed in magnetic resonance imaging (MRI) as contrast agents to enhance image clarity for diagnostic purposes. In drug delivery systems, these microspheres enable targeted therapy by guiding drugs to specific sites in the body using external magnetic fields, minimizing side effects. They are also utilized in cell separation and purification processes, where their magnetic nature allows for efficient isolation of specific cell types from complex mixtures. Additionally, these microspheres play a role in biosensing and immunoassays, improving the sensitivity and accuracy of detecting biological molecules. In environmental applications, they are used for wastewater treatment to remove contaminants through magnetic separation techniques.