Monodispersed magnetite microspheres are widely used in biomedical applications due to their magnetic properties and uniform size. They serve as effective carriers for targeted drug delivery, allowing precise release of therapeutic agents at specific sites in the body, such as tumors. In diagnostics, these microspheres are utilized in magnetic resonance imaging (MRI) as contrast agents to enhance image clarity. They also play a crucial role in magnetic separation techniques, enabling the isolation of specific cells, proteins, or DNA from complex biological samples. Additionally, they are employed in hyperthermia treatment for cancer, where they generate heat under an alternating magnetic field to destroy cancer cells. Their uniform structure and magnetic responsiveness make them valuable in various research and industrial applications, including catalysis and environmental remediation.