Monodispersed magnetite microspheres are widely used in biomedical applications due to their magnetic properties and uniform size. They are employed in magnetic resonance imaging (MRI) as contrast agents to enhance image clarity for diagnostic purposes. In drug delivery systems, these microspheres enable targeted therapy by guiding drugs to specific sites in the body using external magnetic fields, minimizing side effects and improving treatment efficiency. Additionally, they are utilized in cell separation and sorting processes, where their magnetic nature allows for the isolation of specific cell types from complex mixtures. In environmental applications, they are used for wastewater treatment to remove heavy metals and other contaminants through magnetic separation. Their uniformity and stability also make them valuable in research for studying magnetic behaviors and developing advanced materials.