Monodispersed magnetite microspheres are widely used in biomedical applications due to their magnetic properties and uniform size. They serve as effective carriers in targeted drug delivery systems, enabling precise release of therapeutic agents to specific sites in the body, such as tumors. In diagnostics, these microspheres are utilized in magnetic resonance imaging (MRI) as contrast agents to enhance image clarity and improve disease detection. Additionally, they play a crucial role in magnetic separation techniques, where they are employed to isolate and purify biomolecules like DNA, proteins, and cells from complex mixtures. In environmental applications, they are used for wastewater treatment to remove heavy metals and other contaminants through magnetic adsorption. Their consistent size and magnetic responsiveness make them valuable in research and industrial processes requiring high precision and efficiency.