Agarose
Low melting point, suitable for separating small nucleic acid fragments
Reagent
Code: #95907
Alias
agarose; agarose gel, spherical agarose
CAS Number
9012-36-6
blur_circular Chemical Specifications
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Registry Numbers
EC Number
232-731-8
MDL Number
MFCD00081294
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Storage & Handling
Storage
room temperature
description Product Description
Agarose is widely used in molecular biology for gel electrophoresis, a technique that separates DNA, RNA, and proteins based on their size. It forms a porous gel matrix when dissolved in water and allowed to cool, allowing molecules to migrate through it under an electric field. Smaller molecules move faster, while larger ones move slower, enabling scientists to analyze and purify nucleic acids. Agarose gels are also used in techniques like Southern blotting and Northern blotting for detecting specific DNA or RNA sequences. Additionally, it is employed in chromatography for protein purification and in microbiology for culturing certain microorganisms. Its biocompatibility and non-toxic nature make it suitable for various biomedical applications, including drug delivery systems and tissue engineering scaffolds.
format_list_bulleted Product Specification
| Test Parameter | Specification |
|---|---|
| Gelling Range | 26-30 |
| Melting Point 1.5 Gel | 62-68°C |
| Sulfate | <=0.1% |
| Ash Content | <=0.5% |
| Water | 0-10% |
| Appearance | White to off-white powder |
| Solubility (2 g/100 mL water) | colorless to faint yellow, clear |
| Gel Strength | 200 g/cm2 |
| DNase | None detected |
| RNase | None detected |
| Protease | None detected |
| Endonuclease | None detected |
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