Used primarily in the preparation of polyacrylamide gels for electrophoresis, this solution is essential in molecular biology and biochemistry research. It serves as a key component in creating gel matrices that separate proteins or nucleic acids based on size. The ratio of acrylamide to bis-acrylamide determines the gel's pore size, influencing resolution during separation. It is also employed in the fabrication of hydrogels for biomedical applications, such as drug delivery systems and tissue engineering scaffolds, due to its ability to form cross-linked networks with tunable properties. Additionally, it finds use in water treatment processes as a flocculant to remove suspended particles. Proper handling is crucial, as it is a neurotoxin in its monomeric form.