URB-597 is primarily used in research settings to study the endocannabinoid system. It acts as a selective inhibitor of the enzyme fatty acid amide hydrolase (FAAH), which is responsible for breaking down anandamide, a key endocannabinoid. By inhibiting FAAH, URB-597 increases anandamide levels, helping researchers explore its role in pain regulation, mood, and neuroprotection. This compound is particularly valuable in preclinical studies focused on understanding conditions like anxiety, depression, and chronic pain, as well as potential therapeutic applications targeting the endocannabinoid system.