O-2545 hydrochloride is primarily utilized in research settings for studying cannabinoid receptors and their associated pathways. It acts as a potent agonist, making it valuable for exploring the physiological and pharmacological effects of cannabinoid receptor activation. Researchers employ it to investigate potential therapeutic applications, such as pain management, neuroprotection, and anti-inflammatory effects. Additionally, it is used in preclinical studies to understand the role of cannabinoid systems in various diseases, including neurological and psychiatric disorders. Its high selectivity and efficacy make it a critical tool in advancing cannabinoid-related drug development and mechanistic studies.