Used in biomedical research to measure glucocorticoid hormone levels in rat samples. Helps assess stress response, adrenal function, and hypothalamic-pituitary-adrenal (HPA) axis activity in experimental models. Commonly applied in studies involving stress, inflammation, metabolism, and neuroendocrinology. Enables quantitative analysis of glucocorticoids in serum, plasma, or tissue extracts with high sensitivity and specificity. Supports drug development and toxicology studies where hormonal changes are monitored.