Used for the quantitative determination of ecdysone levels in biological samples such as insect hemolymph, tissue extracts, and cell cultures. Commonly applied in entomological research to study molting, development, and metamorphosis in insects. Also utilized in endocrinology and toxicology studies to assess the impact of environmental factors or chemicals on insect hormone regulation. Suitable for high-throughput screening in agricultural research, particularly in evaluating the efficacy of insect growth regulators.