The E-Toxate Reagent derived from Limulus polyphemus is primarily utilized in the detection of bacterial endotoxins. It is a critical component in the Limulus Amebocyte Lysate (LAL) test, which is widely employed in the pharmaceutical and medical device industries to ensure products are free from harmful endotoxins. This reagent reacts specifically with endotoxins, producing a gel clot, color change, or turbidity, depending on the test method used. Its high sensitivity and reliability make it indispensable for quality control in the production of injectable drugs, vaccines, and implantable medical devices. Additionally, it is used in research laboratories to study endotoxin-related immune responses and in environmental monitoring to detect bacterial contamination in water and other samples.