TEPP-46 is primarily used in research settings to study the role of pyruvate kinase M2 (PKM2) in cellular metabolism. It acts as an allosteric activator of PKM2, promoting its tetramerization and enhancing its enzymatic activity. This makes it a valuable tool for investigating cancer metabolism, as PKM2 is often upregulated in tumor cells and contributes to the Warburg effect, a metabolic shift favoring glycolysis even in the presence of oxygen. By modulating PKM2 activity, TEPP-46 helps researchers explore potential therapeutic strategies for targeting cancer cell metabolism. Additionally, it is used in studies related to inflammatory diseases and metabolic disorders, where PKM2 plays a regulatory role in cellular signaling and energy homeostasis. Its application extends to understanding the metabolic adaptations in various pathological conditions, providing insights into disease mechanisms and potential drug development.