The NAD-Malate Dehydrogenase (NAD-MDH) Activity Assay Kit is primarily used to measure the enzymatic activity of NAD-MDH in various biological samples. This enzyme plays a critical role in the tricarboxylic acid (TCA) cycle, facilitating the conversion of malate to oxaloacetate while reducing NAD+ to NADH. The assay is widely applied in research to study cellular metabolism, particularly in understanding energy production and mitochondrial function. It is also utilized in plant biology to investigate photosynthetic processes and stress responses, as NAD-MDH is involved in the malate valve mechanism. Additionally, the kit is employed in clinical studies to assess metabolic disorders and in pharmaceutical research to evaluate the effects of drugs on mitochondrial activity. Its simplicity and sensitivity make it a valuable tool for both basic and applied scientific investigations.