The Transhydrogenase-1 (TH-1) Activity Assay Kit is primarily used to measure the activity of TH-1 enzymes in biological samples. It is widely applied in research focused on cellular metabolism, particularly in studies investigating the redox balance and energy production within cells. This kit is essential for understanding the role of TH-1 in mitochondrial function, as it helps assess the enzyme's ability to transfer reducing equivalents between NADH and NADP+. Researchers utilize this kit to explore metabolic disorders, oxidative stress, and diseases linked to mitochondrial dysfunction, such as neurodegenerative conditions and cancer. Additionally, it is a valuable tool in drug discovery, enabling the screening of compounds that modulate TH-1 activity for therapeutic purposes.