The Transhydrogenase-1 (TH-1) Activity Assay Kit is primarily used to measure the enzymatic activity of TH-1, which plays a critical role in cellular energy metabolism. This kit is widely applied in research settings to study mitochondrial function and redox balance, as TH-1 facilitates the transfer of reducing equivalents between NADH and NADP+. It is particularly useful in investigating metabolic disorders, oxidative stress, and the impact of TH-1 activity on cellular health. Additionally, the kit is employed in drug discovery and development to screen compounds that modulate TH-1 activity, offering insights into potential therapeutic strategies for diseases linked to mitochondrial dysfunction. Its application extends to biochemistry and molecular biology studies, where it aids in understanding the mechanisms of energy production and regulation within cells.