Transhydrogenase-2 (TH-2) Activity Assay Kit is widely used in biochemical research to measure the activity of transhydrogenase-2, an enzyme involved in cellular energy metabolism. This kit is particularly valuable for studying mitochondrial function, as TH-2 plays a critical role in maintaining the balance of NADPH and NADH, which are essential for redox reactions and biosynthetic processes. Researchers utilize this kit to investigate metabolic pathways, oxidative stress, and mitochondrial dysfunction in various diseases, including cancer, neurodegenerative disorders, and metabolic syndromes. It is also employed in drug discovery to screen potential compounds that modulate TH-2 activity, aiding in the development of therapeutic agents targeting mitochondrial-related conditions. The assay provides a reliable, sensitive, and efficient method for quantifying enzyme activity in cell lysates, tissue samples, and purified enzyme preparations.