The Transhydrogenase-2 (TH-2) Activity Assay Kit is primarily used to measure the activity of TH-2 enzymes in biological samples. This kit is essential in research focused on understanding cellular energy metabolism, particularly in the context of NADPH production and its role in redox balance. It is widely applied in studies related to mitochondrial function, oxidative stress, and metabolic disorders. Researchers utilize this kit to investigate the enzyme's role in diseases such as cancer, neurodegenerative conditions, and diabetes, where altered TH-2 activity may contribute to pathological processes. Additionally, it is a valuable tool in drug discovery and development, enabling the screening of compounds that modulate TH-2 activity for therapeutic purposes. The assay is designed for use in various sample types, including cell lysates, tissue homogenates, and purified enzyme preparations, making it versatile for both in vitro and ex vivo studies.