The Cis-Aconitase Activity Assay Kit is primarily used to measure the activity of the enzyme aconitase in biological samples. This enzyme plays a crucial role in the tricarboxylic acid (TCA) cycle, which is essential for cellular energy production. By assessing aconitase activity, researchers can gain insights into mitochondrial function and cellular metabolism. This kit is particularly valuable in studies related to oxidative stress, as aconitase is highly sensitive to reactive oxygen species (ROS). It is widely applied in research involving neurodegenerative diseases, cancer, and aging, where mitochondrial dysfunction is a key factor. Additionally, the kit is useful in toxicology studies to evaluate the impact of environmental toxins or drugs on cellular energy metabolism. Its application extends to both in vitro and in vivo experimental models, making it a versatile tool for understanding metabolic pathways and disease mechanisms.