Used to measure alkaline phosphatase (AP) activity in human biological samples such as serum, plasma, or cell culture supernatants. Commonly applied in clinical diagnostics and research settings to assess liver function, bone metabolism, and placental activity. Helps in detecting conditions like liver disease, bile duct obstruction, and vitamin D deficiency. Also utilized in monitoring disease progression and evaluating treatment efficacy. The kit provides a sensitive and specific method for quantifying AP enzyme levels through immunoassay techniques.