Used in biomedical research to quantify CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase) enzyme activity in human cell or tissue samples. Helps assess de novo pyrimidine synthesis pathway function, which is critical in cell proliferation studies. Commonly applied in cancer research, immunology, and drug development to monitor cellular metabolic changes, evaluate drug effects on nucleotide metabolism, and study diseases involving dysregulated cell growth. The ELISA format allows high-throughput, specific, and sensitive detection in laboratory settings.