Used in biomedical research to detect and quantify caspase-1 (Interleukin-1β Converting Enzyme, ICE) activity in cell lysates or tissue samples. Helps study inflammation-related pathways, particularly in the context of inflammasome activation. Widely applied in immunology, neurodegenerative disease research, and drug development to assess the effectiveness of anti-inflammatory compounds. Provides a reliable, high-throughput method for monitoring protease activity linked to pyroptosis and cytokine maturation.