Used for quantifying human beta-galactosidase (βGAL) enzyme activity in biological samples such as cell lysates, tissue extracts, and serum. Widely applied in research on lysosomal storage disorders, particularly GM1 gangliosidosis, where βGAL deficiency is a key diagnostic marker. Enables monitoring of enzyme replacement therapies and gene therapy efficacy in preclinical and clinical studies. Also utilized in senescence studies, as βGAL activity at pH 6.0 is a biomarker for senescent cells, aiding in aging-related research and cancer therapy investigations. Provides a sensitive, specific, and high-throughput method for detecting functional enzyme levels without the need for radioactive substrates.