Used to measure 5'-nucleotidase (5-NT) enzyme activity in mouse biological samples such as serum, plasma, or tissue homogenates. This ELISA kit enables researchers to quantify 5-NT levels in studies related to purinergic signaling, immune regulation, and vascular function. It is commonly applied in research on inflammation, ischemia-reperfusion injury, and endothelial dysfunction. The assay supports high-throughput analysis with high sensitivity and specificity, making it suitable for preclinical studies involving mouse models of disease.