Used in biomedical research to detect and quantify acid phosphatase enzyme activity in biological samples. Helps assess osteoclast activity in bone metabolism studies and is applied in diagnosing and monitoring diseases involving abnormal phosphatase levels, such as prostate cancer and bone disorders. The ELISA format allows high sensitivity and specificity in measuring ACP concentrations in serum, urine, or cell cultures. Widely used in clinical labs and research settings for enzyme activity analysis.