Used in biomedical research to quantify protein kinase A (PKA) activity in cell lysates, tissue extracts, and biological fluids. Enables investigation of cAMP signaling pathways involved in metabolism, gene expression, and cellular proliferation. Commonly applied in studies of hormone action, neurological disorders, and cancer development. Supports high-throughput screening in drug discovery and evaluation of kinase inhibitor efficacy. Provides a sensitive and specific method for monitoring PKA activation in response to various stimuli.