Used in research settings to quantitatively measure biotinidase enzyme activity in human serum, plasma, or tissue extracts. This ELISA kit enables detection of biotin amidase levels, supporting studies on biotin metabolism, inherited biotinidase deficiency, and related neurological or metabolic disorders. It is particularly valuable in diagnostic research for identifying individuals with partial or profound biotinidase deficiency, which can lead to metabolic dysfunction if untreated. The assay provides a sensitive and specific method for monitoring enzyme levels in clinical samples, aiding in newborn screening follow-up and therapeutic evaluation.