Used in research to measure the activity and expression levels of the CYP7A1 enzyme, which plays a key role in bile acid synthesis. Helps in studying liver function, cholesterol metabolism, and metabolic disorders such as hypercholesterolemia. Commonly used in drug development to evaluate compounds that target cholesterol regulation and in diagnosing disruptions in bile acid pathways. Suitable for ELISA, Western blot, and enzyme activity assays in tissue samples and cell lysates.