Used in neuroscience and biomedical research to quantify acetylcholine transferase enzyme levels in rat samples, aiding in the study of cholinergic neuron function and neurodegenerative diseases such as Alzheimer's. Enables researchers to assess changes in cholinergic activity in response to drugs, toxins, or experimental treatments. The ELISA format allows for sensitive, specific, and high-throughput measurement of ChAT protein in tissue homogenates or cell cultures from rat models.