Used in biomedical research to measure levels of cholesterol 7α-hydroxylase (CYP7A1) in rat samples, this ELISA kit enables the quantification of enzyme activity involved in bile acid synthesis. It is widely applied in studies related to liver function, lipid metabolism, and metabolic disorders such as hypercholesterolemia and fatty liver disease. Researchers use it to assess the impact of drugs, diets, or genetic modifications on cholesterol homeostasis in rat models. The assay provides a sensitive and specific means to monitor CYP7A1 expression, supporting investigations into regulatory pathways of cholesterol breakdown and potential therapeutic interventions.