Used in research settings to quantify DNase-I enzyme levels in biological samples such as serum, plasma, or cell culture supernatants. This assay helps in studying conditions related to impaired DNA clearance, including systemic lupus erythematosus (SLE) and other autoimmune diseases. The kit enables detection of low or dysfunctional DNase-I activity, which is linked to increased circulating cell-free DNA and formation of immune complexes. It is also applied in monitoring disease progression and evaluating therapeutic responses in clinical and preclinical studies. The ELISA format provides a sensitive and specific colorimetric readout, suitable for high-throughput analysis.