Used in biomedical research to quantify levels of human branched chain amino acid dehydrogenase E2 (BCAADE2) in biological samples such as serum, plasma, or cell lysates. This ELISA kit is particularly valuable in studies related to metabolic disorders, especially those involving branched-chain amino acid (BCAA) metabolism, such as maple syrup urine disease (MSUD). It enables researchers to assess enzyme expression and activity indirectly, supporting investigations into mitochondrial function, metabolic regulation, and potential therapeutic interventions. The assay is also applied in clinical research to explore biomarkers associated with liver disease, insulin resistance, and certain neurodegenerative conditions where BCAA metabolism is disrupted.