Used in biomedical research to detect and quantify β-lactamase enzymes produced by human cells or microbes in clinical and laboratory samples. Widely applied in studies of antibiotic resistance, especially to monitor the presence of β-lactamase that breaks down β-lactam antibiotics like penicillins and cephalosporins. Enables researchers to assess bacterial resistance mechanisms, evaluate efficacy of antibiotic inhibitors, and support development of new antimicrobial therapies. Commonly used in microbiology, infectious disease research, and pharmaceutical testing. Provides a sensitive and specific method for measuring enzyme levels in cell cultures, serum, and other biological fluids.