Used in biomedical research to detect and quantify caspase-9, a key enzyme involved in apoptosis (programmed cell death). This kit enables researchers to measure caspase-9 levels in rat tissue or cell samples, helping to study mechanisms of cell death in diseases such as neurodegenerative disorders, ischemia, and cancer. It is commonly applied in preclinical studies to evaluate the effects of drugs or toxins on apoptotic pathways. The assay is useful in immunology, neuroscience, and toxicology research where understanding cellular death regulation is critical.