Used for quantitative detection of human β-glucuronidase in biological samples such as serum, plasma, and cell lysates. Widely applied in research settings to study lysosomal storage disorders, particularly mucopolysaccharidosis type VII (Sly syndrome), where β-glucuronidase activity is deficient. Enables monitoring of enzyme levels in clinical research, evaluation of therapeutic efficacy in enzyme replacement studies, and assessment of gene therapy outcomes. Also utilized in toxicology and drug metabolism studies, as β-glucuronidase plays a role in the breakdown of glucuronide conjugates, influencing drug bioavailability and detoxification processes. Provides a reliable, high-throughput method for enzyme quantification with high sensitivity and specificity.