Used in biomedical research to quantify levels of human 12-lipoxygenase (12-LOX) in biological samples such as cell lysates, tissue extracts, and bodily fluids. This assay helps investigate the role of 12-LOX in inflammatory processes, cardiovascular diseases, diabetes, and cancer. Researchers use the kit to assess enzyme expression and activity under various experimental conditions, supporting studies on oxidative stress and signal transduction pathways. The ELISA format allows for high-throughput, sensitive, and specific detection, making it valuable in both basic science and drug development settings.