Used in biomedical research to quantify levels of methylenetetrahydrofolate reductase (MTHFR) enzyme in human samples. Helps assess enzyme expression and activity linked to folate metabolism, which is important in studying conditions like hyperhomocysteinemia, cardiovascular diseases, neural tube defects, and certain genetic disorders. Widely applied in clinical labs and research settings for enzyme-level analysis without requiring complex instrumentation. Supports studies on MTHFR polymorphisms and their impact on metabolic health.