Used in biochemical and biomedical research to quantitatively measure the activity or expression levels of mitochondrial respiratory chain complex IV in rat tissue samples. This kit is particularly valuable in studies related to mitochondrial dysfunction, oxidative phosphorylation, cellular energy metabolism, and diseases involving mitochondrial impairment such as neurodegenerative disorders, ischemia-reperfusion injury, and aging. By enabling precise detection of complex IV in mitochondrial extracts, it supports investigation into drug toxicity, metabolic diseases, and the effects of therapeutic interventions on mitochondrial health in preclinical rat models.