Used in research to evaluate drug metabolism and enzyme activity in rat liver microsomes. Helps assess the induction or inhibition of mixed functional oxidase enzymes, which play a key role in the biotransformation of xenobiotics, including drugs, toxins, and environmental compounds. Commonly applied in preclinical studies to investigate drug-drug interactions, hepatotoxicity, and metabolic stability. The ELISA format allows for sensitive and specific quantification of MFO components, supporting high-throughput screening in pharmaceutical and toxicological research.