Used in biomedical research to measure monoamine oxidase (MAO) enzyme activity in rat tissue samples, particularly in brain, liver, and mitochondrial preparations. Helps investigate neurological disorders such as depression, anxiety, and Parkinson’s disease by assessing changes in MAO levels. Widely applied in pharmacological studies to evaluate the effects of MAO inhibitors and psychoactive drugs. Enables high-throughput analysis in preclinical trials and toxicology studies. Provides a sensitive and specific method for quantifying MAO protein concentration in experimental models.